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In isopycnic sepa­rations, particle density alone determines final posi­tion during the density gradient. Determine 12-nine compares the sedimentation coefficients and densities of the vari­ety of cellular factors.

If a boundary is x1 centimeters in the axis of rotation at time t1 and x2 centimeters at time t2, then equation 12-12 can be solved by integration as follows. Initial, by transposition,

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The sedimentation coefficient of a particle could possibly be de­termined experimentally employing an instrument often known as an analytical ultracentrifuge. The rotor that spins During this ultracentrifuge ordinarily incorporates two compart­ments. Into a person compartment is put a “reference mobile” that contains sample-cost-free solvent and one other re­ceives a cell made up of the sample for being analyzed.